{"created":"2023-05-15T08:12:34.282361+00:00","id":42,"links":{},"metadata":{"_buckets":{"deposit":"e5ccdbe5-0bf7-4d3d-b15b-7d3836e3144c"},"_deposit":{"created_by":12,"id":"42","owners":[12],"pid":{"revision_id":0,"type":"depid","value":"42"},"status":"published"},"_oai":{"id":"oai:unii.repo.nii.ac.jp:00000042","sets":["9:10:14"]},"author_link":["239","240","241","242","243","244","245","246"],"item_2_biblio_info_9":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2015-03","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"51","bibliographicPageStart":"41","bibliographicVolumeNumber":"6","bibliographic_titles":[{"bibliographic_title":"人間生活学研究","bibliographic_titleLang":"ja"}]}]},"item_2_description_17":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_2_description_45":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Journal Article","subitem_description_type":"Other"}]},"item_2_description_6":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"植物抽出液の手指用洗浄剤としての可能性を見出すことを目的に、廃棄されることの多いキク葉、フキ葉と、抗菌効果が多く報告されている緑茶を試料として、これら植物抽出液の細菌（手指からの分離菌と食中毒菌）に対する抗菌効果を検討した。　植物抽出液の総ポリフェノール量は、いずれの植物抽出液においても抽出水の温度が高くなる程多くなる傾向にあった。また、手指から分離した細菌は平均して総菌数5.1 × 10² 個（log/ml 2.2 ± 0.2）検出された。グラム陽性球菌では、Staphylococcus 属のS. capitis が18 株、S.warneri が1 株、Aerococcus 属のA. urinae が2 株同定され、グラム陰性桿菌では、Serratia 属のSe. plymuthica が3 株同定された。次に、キク葉、フキ葉、緑茶抽出液を抗菌液とし、手指から分離した細菌（S.capitis 41）、及び食中毒菌の標準株E.coli、S.aureus、S.capitis S に対して抗菌試験を行った。その結果、ディスク拡散法により、E.coli、S.aureus に対して菌の生育を阻止するためには、フキ葉、緑茶ともに総ポリフェノール量が22.0mg/ml 程度は必要であることがわかった。また、S.capitis S に対し、フキ葉は0.44mg/ml、緑茶は0.22mg/ml の総ポリフェノール量が必要であることがわかった。なお、キク葉は、本研究で使用した供試菌株に対して抗菌効果を示さなかった。希釈平板法では、いずれの菌株に対しても、フキ葉、緑茶ともに増殖抑制効果があることが明らかになった。また、S.aureus、.capitis S に対して、フキ葉は0.47mg/ml、緑茶は1.0mg/ml 以上で、完全に細菌増殖を抑制できることが明らかになった。一方、ATP 拭き取り検査法により、フキ葉、緑茶の洗浄・消毒効果を検討した結果、ともに飲用濃度、10%濃度で基準値1500RLU を下回った。　以上の結果から、フキ葉、緑茶は、飲用濃度抽出液で手指の洗浄剤として活用可能であることが示唆された。しかし、緑茶では正確なATP 測定が難しいため、ATP 拭き取り検査法以外の細菌培養法などの衛生検査を併用する必要があるといえる。","subitem_description_language":"ja","subitem_description_type":"Abstract"},{"subitem_description":"In order to discover the usability of plant extracts as hand-washing agents, their antibacterial effects against bacteria (those isolated from fingers as well as bacteria associated with food poisoning) were investigated by using the following samples: chrysanthemum leaves and Japanese butterbur leaves, which are often discarded; and green tea, the antibacterial effect of which is frequently reported.Regarding any plant extract, the total amount of polyphenols tended to increase as the temperature of the extract increased. The average count of bacteria isolated from fingers turned out to be the total bacteria count of 5.1 × 10² (log/ml 2.2 ± 0.2). The antibacterial test was conducted on the extracts of chrysanthemum leaves, Japanese butterbur leaves and green tea in order to investigate the effects against bacteria isolated from fingers (Staphylococcus capitis 41) and the standard food-poisoning strains of Escherichi coli (E.coli), Staphylococcus aureus (S.aureus) and Staphylococcus capitis subsp.capitis (S.capitis S ). It was discovered that in order to inhibit the growth of E.coli and S.aureus, the total polyphenols are required at least 22.0 mg/ml for both Japanese butterbur leaf and green-tea extract. It was also revealed that in order to inhibit the growth of S.capitis S, polyphenols are required 0.44 mg/ml for the Japanese butterbur leaf extract and 0.22 mg/ml for the green-tea extract. Moreover, it was revealed that both Japanese butterbur leaf and green-tea extract have growth-inhibition effect against all the strains. It was discovered that 0.47 mg/ml or greater polyphenols of Japanese butterbur leaf extract and 1.0 mg/ml or greater polyphenols of green-tea extract can completely inhibit the growth of S.aureus and S.capitis S. The cleansing and sterilization effects of Japanese butterbur leaf and green-tea extract were investigated through the AIP smear test. The result showed that neither extract reached the standard value of 1500RLU at the concentrations of drinking level and 10%.These findings indicate that it is possible to use Japanese butterbur leaf and green-tea extract as handwashing ]agents at the drinking-level concentration.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_2_publisher_36":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"新潟人間生活学会"}]},"item_2_source_id_10":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1884-8591","subitem_source_identifier_type":"ISSN"}]},"item_2_source_id_12":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA12585279","subitem_source_identifier_type":"NCID"}]},"item_2_version_type_18":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"小山, さくら","creatorNameLang":"ja"},{"creatorName":"オヤマ, サクラ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"辻, 友美","creatorNameLang":"ja"},{"creatorName":"ツジ, トモミ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"永野, 忠聖","creatorNameLang":"ja"},{"creatorName":"ナガノ, タダサト","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"田村, 朝子","creatorNameLang":"ja"},{"creatorName":"タムラ, アサコ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Oyama, Sakura","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tsuji, Tomomi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nagano, Tadasato","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tamura, Asako","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-02-12"}],"displaytype":"detail","filename":"nsk_6_41_51.pdf","filesize":[{"value":"1.8 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"nsk_6_41_51.pdf","url":"https://unii.repo.nii.ac.jp/record/42/files/nsk_6_41_51.pdf"},"version_id":"42675966-d382-4ccb-b7d2-1b5eecbf9d34"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"フキ葉","subitem_subject_scheme":"Other"},{"subitem_subject":"緑茶","subitem_subject_scheme":"Other"},{"subitem_subject":"ポリフェノール","subitem_subject_scheme":"Other"},{"subitem_subject":"抗菌効果","subitem_subject_scheme":"Other"},{"subitem_subject":"ATP","subitem_subject_scheme":"Other"},{"subitem_subject":"Japanese butterbur leaves","subitem_subject_scheme":"Other"},{"subitem_subject":"green tea","subitem_subject_scheme":"Other"},{"subitem_subject":"polyphenols","subitem_subject_scheme":"Other"},{"subitem_subject":"antibacterial effect","subitem_subject_scheme":"Other"},{"subitem_subject":"ATP","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"手指から分離した細菌に対する植物抽出液の抗菌効果","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"手指から分離した細菌に対する植物抽出液の抗菌効果","subitem_title_language":"ja"},{"subitem_title":"Antibacterial effects of the plant extracts against bacteria isolated from fingers","subitem_title_language":"en"}]},"item_type_id":"2","owner":"12","path":["14"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2016-09-08"},"publish_date":"2016-09-08","publish_status":"0","recid":"42","relation_version_is_last":true,"title":["手指から分離した細菌に対する植物抽出液の抗菌効果"],"weko_creator_id":"12","weko_shared_id":-1},"updated":"2023-06-19T05:50:46.920018+00:00"}